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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
15/09/2010 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BATISTA, J. S. DA S.; TORRES, A. R.; HUNGRIA, M. |
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Afiliação: |
JESIANE STEFÂNIA DA SILVA BATISTA, UEL; ADALGISA RIBEIRO TORRES; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Towards a two-dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15: Spotlighting "hypothetical proteins". |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Proteomics, Weinheim, v. 10, n. 17, p. 3176-3189, Sept. 2010. |
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ISSN: |
1615-9861 |
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DOI: |
DOI 10.1002/pmic.201000092 |
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Idioma: |
Português |
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Conteúdo: |
The economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importance of accurate functional annotation of uncharacterized expressed proteins, improving considerably our understanding of the legume-rhizobia symbiosis. MenosThe economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importan... Mostrar Tudo |
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Thesagro: |
Bacteria; Bradyrhizobium Japonicum; Microbiologia; Soja. |
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Thesaurus Nal: |
Microsymbionts; Soybeans. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02370naa a2200241 a 4500
001 1862320
005 2017-08-03
008 2010 bl uuuu u00u1 u #d
022 $a1615-9861
024 7 $aDOI 10.1002/pmic.201000092$2DOI
100 1 $aBATISTA, J. S. DA S.
245 $aTowards a two-dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15$bSpotlighting "hypothetical proteins".
260 $c2010
520 $aThe economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importance of accurate functional annotation of uncharacterized expressed proteins, improving considerably our understanding of the legume-rhizobia symbiosis.
650 $aMicrosymbionts
650 $aSoybeans
650 $aBacteria
650 $aBradyrhizobium Japonicum
650 $aMicrobiologia
650 $aSoja
700 1 $aTORRES, A. R.
700 1 $aHUNGRIA, M.
773 $tProteomics, Weinheim$gv. 10, n. 17, p. 3176-3189, Sept. 2010.
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Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
10/01/2014 |
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Data da última atualização: |
18/02/2014 |
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Autoria: |
EMBRAPA TABULEIROS COSTEIROS. |
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Título: |
Relatório de destaques: 2010-2011-2012. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Aracaju: Embrapa Tabuleiros Costeiros, 2013 |
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Páginas: |
76 p. |
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Descrição Física: |
il. color. |
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Idioma: |
Português |
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Notas: |
Relatório de atividades. |
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Conteúdo: |
Gestão organizacional; Pesquisa & desenvolvimento; Transferência de tecnologia; Comunicação. |
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Palavras-Chave: |
Gestão de P&D; P&D management; P&D Report; Pesquisa agropecuária; Pesquisa e Desenvolvimento; Relatório de pesquisa. |
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Thesagro: |
Agricultura familiar; Instituição de pesquisa; Melhoramento genético animal. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00809nam a2200241 a 4500
001 1980290
005 2014-02-18
008 2013 bl uuuu t 00u1 u #d
100 1 $aEMBRAPA TABULEIROS COSTEIROS.
245 $aRelatório de destaques$b2010-2011-2012.
260 $aAracaju: Embrapa Tabuleiros Costeiros$c2013
300 $a76 p.$cil. color.
500 $aRelatório de atividades.
520 $aGestão organizacional; Pesquisa & desenvolvimento; Transferência de tecnologia; Comunicação.
650 $aAgricultura familiar
650 $aInstituição de pesquisa
650 $aMelhoramento genético animal
653 $aGestão de P&D
653 $aP&D management
653 $aP&D Report
653 $aPesquisa agropecuária
653 $aPesquisa e Desenvolvimento
653 $aRelatório de pesquisa
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